Apr 07, 2015 · 2.3. Gas Chromatography. GC-FID: a GC-2010 system (Shimadzu), with an SLB-5ms column (Supelco/Sigma-Aldrich, Bellefonte, PA, USA), 30 m 0.25 mm I.D. 0.25 μm d f, was used. For A. bisporus analysis, a different column stationary phase was used: Supelcowax-10, 30 m 0.25 mm I.D. 0.25 μm d f. Oven temperature program was 40°C at 5°C/min to 250