To better understand both the mechanism of collagenase during infection and facilitate its use in drug delivery, the high-resolution structures of the PKD-like domains and CBD of collagenases ColG and ColH were solved using X-ray crystallography. The structures of Ca2+-absent (apo)-s2 and s2a, as well as Ca2+-bound (holo)-s2a, s2b, s3a-s3b, and