Head space gas chromatography was selected to analyze acetic butyric acid. The equipment used was an Varian ProsStar GC-3800, Varian, Inc., CA, USA fitted with flame ionization detector (FID), connected with a hydrogen generator (UHP-20H NITROX, Domnick Hunter Ltd., Gateshead England, UK) and equipped with a Nukol, fused silica high-quality coated polyamide capillary column 15 m × 0.32 mm I.D