Mar 19, 2014 · Blank blood sample Pipette 1 ml blank whole blood confirmed with no ethanol, into the 20 ml headspace vial, cover the vial with a polytetrafluoroethylene film, silastic seal, and then pressurize the aluminum cap with a crimper, seal, and mix well. Heat the vials in the headspace inlet for 10 min at 60°C.