Nov 07, 2014 · Liquid chromatography was performed by means of a micro HPLC/autosampler/vacuum degasser system PE Series 200 (Perkin Elmer, Norwalk, CT). The analytes were separated on a X-Terra MS C18-column (4.6 × 150 mm, 5 μm, Waters Corporation, USA) protected by a guard column of the same type (4.0 × 10 mm, 5 μm, Waters Corporation, USA).