Afterwards, aliquots of 3 g of blanched carrots were placed in 20 ml gas-tight vials and hermetically sealed with a gas-tight septum. A total of 24 vials were prepared for each temperature tested (−18 °C, 4 °C, 25 °C, 40 °C), in order to have three replicates (three different vials) for each day of measurement (1, 4, 8, 12, 19, 26 days).